Evol Ecol Res 13: 91-104 (2011) Full PDF if your library subscribes.
Spatial and temporal analysis of the genetic diversity and population structure of a freshwater ostracod from the high Andean plateau
Rodrigo Scheihing1,2, Leyla Cardenas3, Pedro Labarca1 and Roberto F. Nespolo3
1Centro de Estudios Científicos (CECS), Valdivia, Chile, 2Escuela de Graduados and 3Instituto de Ecología y Evolución, Universidad Austral de Chile, Valdivia, Chile
Correspondence: R. Scheihing, Centro de Estudios Científicos (CECS), Castilla 1469, Valdivia 5110246, Chile.
Background: The endorheic basins (basins with no outflow to another body of water) of the Andes Mountains contain several populations of small invertebrates with varying levels of isolation. Little is known about their genetic composition.
Hypothesis: Population isolation and changes in population size over time will increase genetic diversity.
Organisms: Three populations of the ostracod Limnocythere sp. with varying levels of isolation and population stability.
Field site: El Monumento Natural Salar de Surire, Chile, in the high Andean Plateau at 4200–4300 m above sea level.
Methods: We surveyed two molecular markers: the cytochrome oxidase I (COI) region and the internal transcribed spacer (ITS) region. With these data, we estimated the genetic diversity and spatial genetic structure during four consecutive years.
Conclusions: Genetic structure within populations did not differ among years, although there were differences among populations.
Keywords: population, molecular markers, gene flow, environmental stochasticity, ostracod, high Andean plateau.
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