Evol Ecol Res 17: 125-140 (2016) Full PDF if your library subscribes.
in the surface layer of sediments from the East China Sea
Ran Jiang*, Jian-Xin Wang*, Kai-Cheng Yu, Ming-Hua Liu, Ge Shi and Xue-Zhu Liu
Marine Microorganism Ecological and Application Lab, Zhejiang Ocean University, Zhejiang, China
Correspondence: Jian-Xin Wang or Xue-Zhu Liu, Marine Microorganism Ecological and Application Lab, Zhejiang Ocean University, Number 1 South Haida Road, Zhejiang 316022, China.
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Question: What is the micro-eukaryotic diversity in the surface layer of sediments from the East China Sea?
Methods: We sequenced 332 clones from surface sediments at three different sites, and then selected 129 representative phylotypes for phylogenetic analysis. We used culture-independent molecular approaches based on 18S rRNA genes to investigate the diversity. We also used redundancy analysis to determine the relationship between sediment microbial community diversity and environmental parameters.
Results: Protists were highly diverse. Most clone sequences were affiliated to protists (72.6%), dominated by Alveolata (57.2%) and Rhizaria (12.0%). The other clone sequences belonged to Metazoa (17.6%), Stramenopiles (3.4%), fungi (2.6%), Apusozoa (2.1%) and ‘unclassified’ (1.7%). A number of the clones were related to anoxic environment clones or polar region clones. Redundancy analysis showed that some environmental parameters had a marked effect on the sediment microbial community diversity.
Keywords: marine sediment, micro-eukaryotic, RDA, 18S rRNA.
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